Fig. 7.

The expression of NCKX transcripts revealed by RT-PCR (A) and in situhybridization (B). A, Total RNAs were extracted from rat brain SON and reverse-transcribed [RT(+)] using reverse transcriptase plus taq polymerase PCR amplification of cDNAs was performed using a specific set of primers for NCKX2 and NCKX3. RT-PCR demonstrates expression of mRNA for NCKX2 and NCKX3 in SON. DNA contamination was tested from the absence of PCR product without reverse transcriptase [RT(−)]. B, C,In situ hybridization using digoxigenin-labeled NCKX2 (B) and NCKX3 (C) antisense riboprobes was performed on the coronal sections of the rat brain. In all cases, the sense control resulted in no increase in contrast above the general background observed in the panels of this figure.Ba, SON; Bb, PVN; Ca, SON;Cb, thalamus (Thal). The regions corresponding to the optic tract (OT) and the third ventricle (III) are marked inBa and Bb, respectively. Scale bar, 100 μm.