Fig. 3.

The retrograde transport of transgene-derived GDNF in motoneurons of the spinal cord. At 110 d of age (7 weeks after vector administration), transgene-derived GDNF was detected in the ventral horn of the corresponding spinal cord ipsilateral to the AAV-GDNF vector-injected side on double-immunofluorescence staining with anti-FLAG (a) and SMI-32 (b) antibodies. Merging of a and b showed colocalization of FLAG and SMI-positive neurons in the anterior horns, suggesting that the retrograde transport of transgene-derived GDNF occurred in motoneurons (c). d–f, The contralateral side injected with AAV-LacZ vector. Scale bar, 20 μm.