Fig. 5.
Protein kinase C isoforms in rat SCG and their role in bradykinin-evoked transmitter release. A,Lysates of rat cortex (C) or SCG cell cultures were separated by SDS-PAGE, transferred to nitrocellulose membranes, and stained with antibodies directed against the indicated subtypes of PKC. Approximate positions of molecular weight markers are indicated.B, Before SDS-PAGE, SCG cultures were treated with 1 μm PMA for the indicated periods of time. The blots were stained with antibodies directed against the indicated subtypes of PKC.C, Cultures were treated with either 1 μmPMA or 1 μm 4-α-phorbol for 24 hr, labeled with [3H]noradrenaline, and superfused. Subsequent to a 60 min washout period, 4 min fractions of superfusate were collected, and 1 μm bradykinin was present for 2 min, as indicated by the bar. The graphs show the time course of fractional [3H] outflow calculated as percentage of the total radioactivity (% of TA) in the cells; n = 6.