Fig. 5.

Identification in the LIVBP-like domain of NR2B critical residues controlling high-affinity ifenprodil inhibition. A comparison of the current traces obtained from oocytes coexpressing NR1 with either wt or mutated NR2B subunits is shown. Ifenprodil was applied at two increasing concentrations (300 nm and 3 μm) during an application of agonists. Thebars above the current traces indicate the duration of agonists (agos) and ifenprodil (ifen) applications. The holding potential was −60 mV. These current traces are typical of NR2B mutants having an intermediate (V42A) or strong (D101A, F176A) effect on ifenprodil sensitivity.