Fig. 6.

Ifenprodil concentration–response curves of receptors mutated at critical residues. Each graphcorresponds to a region in the LIVBP-like domain of NR2B in which one or more critical residues controlling ifenprodil inhibition were identified. The dotted curves are the fits of the ifenprodil concentration–response curves of the NR1/NR2B wt receptors (left dotted curve) and the chimeric NR1/NR2B-(LIVBP NR2A) receptors (right dotted curve) obtained in Figure2B. The estimated values of IC₅₀ of the different mutated receptors are listed in Table 2. Estimated values of Hill coefficients (0.92–1.10) and maximal inhibitions (88–100%) are in the same range as those obtained with NR2B wt receptors (Fig.2A). Each data point is the mean value obtained from 3 to 22 oocytes. The recordings were made at −60 mV.