Table 1.

Yeast two-hybrid interactions of the NF-M clone with the third cytoplasmic loops of all dopamine receptors

DA receptors	Gal/Raf-H-U-W-L NF-M	Gal/Raf-H-U-W, X-Gal NF-M
D1	Yes	3
D2	No	0
D3	No	0
D4	No	0
D5	Yes	1
D5-N terminus	Yes	2
D5-C terminus	No	0
D1-N terminus	Yes	2
D1-C terminus	Yes	2
LexA	No	0
Positive control	Yes	4

Bait proteins were constructed, and yeast two-hybrid assays were performed as described in Materials and Methods. Bait proteins labeled “N terminus” and “C terminus” refer to the N- and C-terminal portions of the third cytoplasmic loops of the D₁ and D₅ receptors. “Yes” refers to growth of yeast in media lacking histidine, uracil, tryptophan, and leucine (Gal/Raf-H-U-W-L). Numbers denote the relative intensity of “blue” color detected in media containing X-gal. Negative (LexA) and positive controls are shown. The positive control represents the yeast two-hybrid result using bait and prey plasmids expressing the P53 and LTA interacting proteins. This positive control provided the “bluest” color change and was arbitrarily assigned the number 4.