Table 1.

Role of F-actin in ephrin-A2 induced endocytosis

Treatment	Number of dextran-labeled vesicles (n)
Vehicle (DMSO)	4.9  ± 0.5 (84)
Cytochalasin D	5.5  ± 0.6 (82)
Latrunculin-A	4.3  ± 0.5 (89)
Vehicle + ephrin-A2	11.1  ± 0.9 (76)*
Cytochalasin D + ephrin-A2	10.1  ± 0.8 (87)*
Latrunculin-A + ephrin-A2	9.1  ± 0.8 (80)*

Retinal growth cone collapse induced by F-actin depolymerizing drugs did not increase endocytosis compared with vehicle alone (p > 0.05). Addition of ephrin-A2 resulted in a significant increase in endocytosis in all conditions examined.

^*Significant differences compared with identical cultures without ephrin (p < 0.001).