Fig. 1.
Analysis of the proportion of the neuronal phenotype in cultured cerebral cortical cells. A, Fluorescent immunolabeling of the neuronal marker, NSE, in paraformaldehyde-fixed cortical cells obtained from the frontal lobe of E15 mouse fetuses and cultured for 3 d. B, The same cells stained with BZM, which is a fluorescent stain for cell nuclei. Scale bar, 30 μm. C, Histograms showing the densities of NSE-immunolabeled neuronal cell bodies and BZM-stained nuclei in cortical cell cultures. Each bar represents the mean ± SEM of seven separately generated cultures. Note that ∼97% of all nuclei in these cultures belong to NSE-expressing cell bodies.