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. 2002 Sep 1;22(17):7408–7416. doi: 10.1523/JNEUROSCI.22-17-07408.2002

Fig. 4.

Fig. 4.

MIT toxicity and MIT-induced ERK activation requires 12-LOX activity. A, MIT (100 μm) toxicity was significantly inhibited by the 12-LOX inhibitor baicalein (20 μm) and by the less-specific LOX inhibitor AA861 (1 μm). Results represent the mean ± SEM (n = 3); ∗∗∗p < 0.001.B, Immunoblots demonstrate that MIT-induced ERK activation is blocked by baicalein. Baicalein had no effect on ERK activation when added alone (data not shown). Similar results were observed in a total of three independent experiments.Phospho-ERK, Phosphorylated ERK.C–F, 12-LOX immunostaining in control cultures (C) and 5 min after a 10 min exposure to 100 μm MIT alone (D) or in the presence of either 20 μm baicalein (E) or 1 μm TPEN (F). LOX activation is usually accompanied by its translocation to the cell membrane.