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MIT induces a decrease in GSH activity. Cultures were exposed to 100 μm MIT for 10 min and assayed for cellular GSH activity 5 min later. MIT induces a decrease in GSH levels, which can be prevented by coexposure to the cysteine reagent NAC (1 mm) but not by the antioxidant trolox (100 μm) or by TPEN (1 μm) (mean ± SEM;n = 3–4); ∗∗p < 0.01.
