Fig. 7.

NADPH oxidase inhibitors are protective against MIT toxicity. A, MIT (100 μm) toxicity was significantly inhibited by the NADPH inhibitors AEBSF (100 μm) and DPI (100 nm). Results represent the mean ± SEM (n = 3); ∗p< 0.05. B, Immunoblots demonstrating that MIT-induced ERK activation was not blocked by AEBSF or DPI. Neither of these compounds had an effect on ERK phosphorylation when added alone (data not shown). Similar results were observed in a total of three independent experiments. Phospho-ERK, Phosphorylated ERK.