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. 2002 Sep 1;22(17):7478–7484. doi: 10.1523/JNEUROSCI.22-17-07478.2002

Fig. 1.

Fig. 1.

A P-element-mediated endophilin mutant shows an activity-dependent loss in synaptic transmission in the eye.A, Drosophila eye bright-field images depicting the original eyeless stock followed by the rescued eye clones (Ey Gal4 UAS Flp;GMR-Hid FRT 82B/EP0927 FRT 82B) and the mutant eye clones (Ey Flp;GMR-Hid FRT 82B/EP0927 FRT 82B) (see Results for details). B, ERG recordings from wild-type, rescued, and mutant clones. Stimulation with a light pulse at 2 Hz (i) followed by a heightened 10 Hz stimulation for 1 min (ii) and immediate ERG recording (iii) is shown. Finally, flies were dark-adapted for 15 sec, and the ERG was recorded (iv). Solid arrows point to transients and open arrowheads point to a loss of transients. C, Phototaxis for wild-type flies with endophilin-rescued clones and mutant clones, respectively (n = 3 in each case). The PhI is indicated on they-axis. Error bars indicate SD. d, Semiquantitative RT-PCR for endophilin transcript levels from the heads of adult wild-type and mutant eye clones. CS, Canton Special; Rp49, concentration control for mRNA.