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. 2002 Nov 1;22(21):9340–9351. doi: 10.1523/JNEUROSCI.22-21-09340.2002

Fig. 2.

Fig. 2.

Immunoblot analysis of tau protein in brains and spinal cords from mice of the human P301S tau line. A, Perchloric acid-soluble tau was extracted from the brain (B) and spinal cord (SC) of 5- to 6-month-old mice and immunoblotted with anti-tau antibodies BR134 and T14 before (−) and after (+) alkaline phosphatase treatment.Black arrowheads point to the three mouse tau isoforms, with the white arrowhead pointing to the single human tau isoform. B, Sarkosyl-insoluble tau was extracted from brains (B) and spinal cords (SC) of 5- to 6-month-old transgenic mice and immunoblotted with T14 before (−) and after (+) alkaline phosphatase treatment. M, Mixture of the six recombinant human brain tau isoforms. C, Reactivities of perchloric acid-soluble and sarkosyl-insoluble tau with a panel of 10 different phosphorylation-dependent anti-tau antibodies.