Fig. 4.

Effect of microglia-conditioned medium (MCM) on trophic factor expression in cultured Müller glial cells. A, Experimental protocol for examining the effect of MCM prepared from either normal or light-damaged P35 rat retina. Müller cells were incubated with MCM for 12 hr, and mRNA levels of trophic factors were determined by quantitative PT-PCR. B–G, RT-PCR analysis of NGF (B), BDNF (C), NT-3 (D), CNTF (E), bFGF (F), and GDNF (G). Note the upregulation of BDNF (C) and downregulation of bFGF (F) in Müller cells after incubation with light-reared MCM. Each data point represents the mean ± SEM of the values obtained from three independent experiments. *p < 0.01.