Fig. 5.
Effect of microglia-conditioned medium (MCM) on bFGF expression in cultured Müller cells from p75NTR knock-out mice. A, Experimental protocol for examining bFGF mRNA levels in Müller cells from wild-type (p75+/+) and p75NTR knock-out (p75−/−) mice. Müller cells were incubated with MCM for 12 hr, and bFGF mRNA levels were determined by quantitative RT-PCR. B, RT-PCR analysis of bFGF. Note the stable bFGF expression levels in Müller cells from p75NTR knock-out (p75−/−) mice. Each data point represents the mean ± SEM of the values obtained from three independent experiments. *p < 0.01.