Table 1.
Summary of ultrastructural analysis
| Genotype | +/+ | pabpEP0310/Df(2R)Pcl7b | DGluR-IIA-ko |
|---|---|---|---|
| Number of branches analyzed | 4 | 5 | 16 |
| Number of scored ultrathin sections (∼87 nm) | 244 | 243 | 520 |
| Covered length of NMJ (μm) | 21.2 | 21.1 | 45.2 |
| Number of scored synaptic profiles | 628 | 561 | 872 |
| Number of scored T-bar profiles | 126 | 185 | 468 |
| Mean terminal area (μm2)/section | 0.8 ± 0.3 | 0.9 ± 0.3 | 0.5 ± 0.1 |
| Number of synaptic profiles/section | 2.6 ± 0.9 | 2.3 ± 0.7 | 1.7 ± 0.3 |
| Number of T-bar profiles/section | 0.5 ± 0.4 | 0.8 ± 0.5 | 0.9 ± 0.3 |
| Number of synaptic profiles/terminal area | 3.1 ± 0.6 | 2.6 ± 0.2 | 3.7 ± 0.3 |
| Number of T-bar profiles/terminal area | 0.6 ± 0.1 | 0.7 ± 0.1 | 1.9 ± 0.2 |
| Number of T-bar profiles/synaptic profile | 0.21 ± 0.02 | 0.25 ± 0.05 | 0.53 ± 0.03 |
| Relative density of synapses (%) | 100 ± 19 | 84 ± 6 | 119 ± 10 |
| Relative density of active zones (%) | 100 ± 21 | 108 ± 23 | 310 ± 24 |
Summary of scored and extrapolated data obtained from randomly sampled ultrathin sections of type Ib boutons at larval NMJs ofDrosophila (abdominal segment 2; see Materials and Methods). Data were collected and treated separately for each identified type Ib branch. Values represent branch means ± SEM. The relative densities of synapses and T-bars are equivalent to the numbers of synaptic profiles and T-bar profiles per terminal area, respectively, in which the wild-type (+/+) values are set to 100%.