Skip to main content
. 2002 Sep 15;22(18):7913–7922. doi: 10.1523/JNEUROSCI.22-18-07913.2002

Fig. 4.

Fig. 4.

Kv1.4 subunits with inserted Src family SH3 domain ligand sequence (Kv1.4-N-Pro and Kv1.4-C-Pro) precipitate from lysates of transfected HEK 293 cells by purified bacterially expressed Src SH3 domains. A, α-Kv1.4 Western blots of lysates and Src SH3 domain precipitates from HEK 293 cells transfected with Kv1.4-wt, Kv1.4-N-Pro, or Kv1.4-C-Pro. Blots are representative of three experiments. B, α-Kv1.4 Western blot of wild-type Src SH3 domain (wt) and D99N point mutant Src SH3 domain (ko) precipitates from HEK 293 cells transfected with Kv1.4-wt, Kv1.4-N-Pro, or Kv1.4-C-Pro. Both short and long exposures are shown. Identical amounts of Src SH3 fusion protein were added to, and precipitated from, each precipitation reaction (data not shown). The blot is representative of four experiments.