Fig. 5.

PSD95β and PSD95α synaptically cluster in primary hippocampal neurons. Constructs encoding PSD95β–GFP or PSD95α–GFP were transfected into neurons that were then fixed and stained for synaptophysin (red). After 11–17 din vitro, PSD95α–GFP (A–C) and PSD95β–GFP (D–F) cluster at synaptic sites. PSD95β–GFP lacking the N-terminal 19 residues [PSD95β(Δ1–19)–GFP] retains synaptic localization (G–I), whereas deleting the N-terminal 44 residues from [PSD95β-Δ(1–44)–GFP] disrupts synaptic targeting (J–L). Merged images are shown in panels onright (C, F, I, L). Scale bar, 10 μm.