Table 1.

Statistical summary

	ANOVAs				Tukeypost hoc
	Effects	DF	F	p	Groups	p
Figure2	Tx	1,23	6.442	0.006	1 versus 2	0.004
	Time	3,69	34.764	<0.001	1 versus 3	0.384
	Time × tx	6,69	0.444	0.778	2 versus 3	0.049
Figure3	Tx	1,20	0.740	0.400
	Time	3,60	0.944	0.393	n/a*
	Time × tx	3,60	0.143	0.854
Figure4	Tx	3,32	1.139	0.348
	Time	3,96	6.040	0.004	n/a*
	Time × tx	9,96	1.413	0.224
Figure5a					1 versus 2	0.016
					1 versus 3	0.006
	Tx	3,24	6.614	0.002	1 versus 4	0.967
	Time	3,72	0.063	0.951	2 versus 3	0.833
	Time × tx	9,72	1.160	0.941	2 versus 4	0.133
					3 versus 4	0.045
Figure5b	Tx	2,20	7.626	0.003	1 versus 2	0.003
	Time	3,60	9.781	<0.001	1 versus 3	0.642
	Time × tx	6,60	1.084	0.379	2 versus 3	0.021
Figure 5c	Tx	2,19	31.321	<0.001	1 versus 2	<0.001
	Time	3,57	0.229	0.732	1 versus 3	<0.001
	Time × tx	6,57	0.571	0.638	2 versus 3	0.959
Figure 6(DA)	Tx	1,12	0.486	0.499
	Time	5,60	3.379	0.040	n/a*
	Time × tx	5,60	3.133	0.050
Figure 6(JOR)	Tx	1,7	0.018	0.896
	Time	5,35	1.528	0.249	n/a*
	Time × tx	5,35	0.095	0.192
Figure 7(DA)	Tx	1,9	16.512	0.003
	Time	5,45	2.195	0.161	n/a*
	Time × tx	5,45	2.344	0.147
Figure 7(JOR)	Tx	1,9	42.717	<0.001
	Time	5,45	0.900	0.431	n/a*
	Time × tx	5,45	0.402	0.693

The discussion and conclusions of this study are based primarily on the main effect of treatment (Tx) and the Tukey post hocanalyses shown in the extreme right column; however, the main effect of time (Time) and the time × treatment interaction (Time × tx) are shown for completeness. The identity of the groups in thepost hoc column is indicated by the numbers that are given in each of the respective figures. In some experiments, the JOR was measured in parallel with the collection of microdialysis fractions. Figure 6 (DA) and Figure 7 (DA) show the statistical analyses of dopamine release induced by capsaicin or morphine, respectively (shown in Figs. 6 and 7). Figure 6 (JOR) and Figure 7 (JOR) show the statistical analyses for the JOR measurements taken in parallel with the microdialysis samples; some microdialysis experiments were done without simultaneous JOR measurements. See Figures 2 and 3 for the effect of morphine or capsaicin, respectively, on the JOR in morphine naïve and tolerant rats.

*Post hoc analyses were not done because there were only two groups or because there was no significant main effect of treatment (i.e., Fig. 4).