Table 1.

Effect of ET-1 on activation and inactivation of TTX-R and TTX-S sodium channels in rat DRG neurons

	Activation curve*		Availability curve**
	E0.5(mV)	ka (mV)	h0.5(mV)	kh (mV)
TTX-R
Control cells (n = 6)
Baseline	−14.49 ± 1.29	5.59 ± 0.72	−24.75 ± 0.44a	4.76 ± 0.02
10 min	−16.67 ± 1.35	6.13 ± 0.70	−25.67 ± 0.53	5.29 ± 0.00
20 min	−14.11 ± 0.87	5.95 ± 0.67
Δ[10 min − baseline]	−2.17 ± 0.42	1.03 ± 0.54	−0.92 ± 0.09	0.53 ± 0.02
ET-1 responsive cells (n = 10)
Baseline	−18.94 ± 1.42	5.39 ± 0.25	−27.26 ± 1.48	5.33 ± 0.10
ET-1	−26.98 ± 1.34	4.64 ± 0.27	−32.44 ± 1.50	6.02 ± 0.29
Δ[ET-1 − baseline]	−8.04 ± 0.54	0.75 ± 0.27	−5.18 ± 0.37	0.69 ± 0.26
ET-1 non-responsive cells (n = 7)
Baseline	−13.33 ± 2.20	5.93 ± 0.45	−20.57 ± 0.35b	5.33 ± 0.03
ET-1	−15.58 ± 2.34	4.64 ± 0.27	−22.81 ± 0.38	5.93 ± 0.04
Δ[ET-1 − baseline]	−3.25 ± 0.18	0.07 ± 0.17	−2.24 ± −0.03	0.60 ± 0.03
BQ-123 plus ET-1-treated cells (n = 10)
Baseline	−10.27 ± 1.85	6.67 ± 0.36	−23.55 ± 1.85	6.41 ± 0.34
BQ-123	−12.34 ± 1.84	5.94 ± 0.37	−25.12 ± 2.02	6.51 ± 0.56
BQ-123 plus ET-1	−13.93 ± 1.90	6.13 ± 0.24	−29.05 ± 2.11	6.63 ± 0.38
Δ[BQ-123 − baseline]	−2.07 ± 0.61	1.54 ± 0.45	−1.85 ± 0.50	0.13 ± 0.30
Δ[(BQ-123 + ET-1) − BQ-123]	−1.54 ± 0.45	0.19 ± 0.17	−2.90 ± 0.35	0.07 ± 0.26
TTX-S (n = 7)
Baseline	−14.49 ± 1.29	5.59 ± 0.72	−24.75 ± 0.44	4.76 ± 0.02
ET-1	−16.67 ± 1.35	5.95 ± 0.67	−25.67 ± 0.53	5.29 ± 0.01
Δ[ET-1 − baseline]	−2.17 ± 0.42	1.03 ± 0.54	−0.92 ± 0.09	0.53 ± 0.02

All values listed are means ± SEM. *Boltzmann equation for fitting the activation curve is as follows:G/G_max = {1 + exp[−(E −E_0.5)/k_a]}⁻¹. **Boltzmann equation for fitting the availability curve is as follows:I/I_max = (A_max− A₀)/{1 + exp[(h_0.5 −E)/k_h]} +A₀.

^F1-an = 4.

^F1-bn = 4.