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. 2002 Aug 1;22(15):6447–6457. doi: 10.1523/JNEUROSCI.22-15-06447.2002

Fig. 2.

Fig. 2.

Analysis of transgene mRNA expression. Expression of the mutant AChR mRNA in the panel of transgenic mice was comparable between several lines and well above that of endogenous mouse AChR subunit genes. A, Matched expression of AChR subunit transgenes. Top panel, Ten micrograms of total muscle RNA per mouse were hybridized with common sequence probe for all transgenes. Exposure was 4 d. Bottom panel, Same blot rehybridized with MCK cDNA to ensure uniform loading. Exposure was 4 hr. Lane 1, δS262T, line 40; lane 2, αC418W, line 42; lane 3, αL251T, line 39;lane 4, αL251T, line 36; lane 5, εL269F, line 14; lane 6, εL269F, line 5; lane 7, control muscle. B, Overexpression of mutant α subunits. Top panel, Total muscle RNA probed with mouse α subunit cDNA. Exposure was 5 d. Endogenous α subunit mRNA is undetectable at this explosure time. Lane 1, αC418W, line 42; lane 2, αL251T, line 39;lane 3, αL251T, line 36; lane 4, control muscle. Bottom panel, Same blot reprobed with MCK cDNA. Exposure was 4 hr. Size markers are in kilobases.