Fig. 2.
TRPV4 mediates heat-evoked calcium influx in HEK 293 cells. A, Relative [Ca2+]i (indicated by ratio of fura-2 emission at 340/380 nm excitation) in HEK 293 cells transiently transfected with TRPV4 or control vector (four each) after a reduction in osmolarity from 290 to 250 mOsm (horizontal bars) at 24°C. B, C, Relative [Ca2+]i in HEK 293 cells transiently (B) or stably (C) transfected with TRPV4 or control vector (four each) during a heat stimulus from 24 to 40°C in ∼40 sec. D, Comparison of heat-evoked (40°C) increases in fura ratio among cells transiently transfected with control vector (P;n = 5), TRPV4 (V4;n = 12), TRPV1 (V1;n = 3), Drosophila TRPL (dT; n = 3), or human TRPC3 (hT; n = 4) or among stable transformants generated with control vector (P;n = 8), TRPV4 (V4;n = 15), or TRPV1 (V1;n = 13). Data represent the mean ± SEM of the indicated number of coverslips. Comparisons are with controls. *p < 0.05; ***p < 0.001;NS, not significant; unpaired ttest.