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. 2002 Jul 1;22(13):5310–5320. doi: 10.1523/JNEUROSCI.22-13-05310.2002

Fig. 4.

Fig. 4.

Comparison of pharmacological characteristics of VIP induction by PACAP and the combination of KCl and forskolin.A, D600 and ascomycin block VIP peptide induction by PACAP and combined KCl and forskolin. Chromaffin cells were pretreated for 30 min with 30 μm D600 or 0.1 μmascomycin (ASCO), followed by treatment with 100 nm PACAP-27 or a combination of 40 mm KCl and 25 μm forskolin (FOR). Vehicle (0.1% ethanol in culture medium) was identical for all conditions. Cells were harvested 72 hr later for VIP radioimmunoassay as described in Materials and Methods. *Different from corresponding control,p < 0.0001; #different from corresponding treatment without inhibitor, p < 0.0001; Scheffe's post hoc analysis. B, D600 and ascomycin block VIP mRNA induction by PACAP and combined KCl and forskolin. Experimental conditions are as described forA, except that RNA extracts were prepared 18 hr after addition of PACAP or KCl plus forskolin, as described in Materials and Methods. C, Effect of D600 on induction of VIP by forskolin and KCl. VIP peptide levels were measured 72 hr after addition of 25 μm forskolin or 40 mm KCl. Either 10 or 30 μm D600 or vehicle was added 30 min before addition of forskolin, KCl, or vehicle. *Different from corresponding control; p < 0.0001;# different from corresponding treatment without inhibitor; p < 0.0001; Scheffe's post hoc analysis.