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. 2002 Jul 1;22(13):5310–5320. doi: 10.1523/JNEUROSCI.22-13-05310.2002

Fig. 6.

Fig. 6.

Stimulation of VIP biosynthesis by 100 nm PACAP and forskolin does not involve protein kinase A activation. A, H89 does not inhibit PACAP induction of VIP. Chromaffin cells were pretreated for 30 min with 10 μm H89 followed by treatment with 10 nmPACAP-27. Vehicle (0.1% ethanol in culture medium) was identical for all conditions. Cells were harvested 72 hr later for VIP radioimmunoassay as described in Materials and Methods. *Different from vehicle; p < 0.0001; Scheffe's post hoc analysis. B, H89 inhibits PKA activity in chromaffin cells. Chromaffin cells were cotransfected with pFR-Luc (reporter), pFA2-CREB, and pFC-PKA (PKA) or pFC2-dbl (control) in the presence or absence of 10 μmH89 as described in Materials and Methods. Cells were harvested 36 hr later for measurement of luciferase activity. *Different from PKA/H89;p < 0.0001; Scheffe's post hocanalysis.