Fig. 1.

A, Polygraph recordings used to identify and quantify states of sleep and wakefulness. Eachpanel shows a 60 sec recording obtained from the same cat. B, ACh chromatograph peaks obtained using microdialysis and HPLC/EC. Representative ACh samples collected during Ringer's solution dialysis (Control) and during dialysis with Ringer's solution containing NLA are shown for wakefulness (left), NREM sleep (middle), and REM sleep (right). Note the increase in peak height, indicating an increase in amount of ACh (in picomoles) caused by dialysis administration of NLA. Resp, Respiration;LGB, lateral geniculate body.