Fig. 1.
Effect of JB-1 infused into the third ventricle on E2-induced α1B-adrenoceptor (AR) density in the POA and HYP. Membranes from POA and HYP were prepared from OVX control and E2-treated (EB) female rats infused with saline or JB-1 as described in Materials and Methods. A, Total3H-prazosin binding corrected for nonspecific binding reflects both α1-AR subtypes. B, The α1A-AR population is measured after CEC inactivation.C, The α1B-AR population is determined by subtracting the binding of 3H-prazosin after CEC inactivation from total 3H-prazosin binding.Bmax values were obtained by Scatchard analysis. The data presented are the means ± SEM from four independent replications. *p < 0.05.