Fig. 6.

Dependence of CaMKII translocation but not NMDAR translocation on filamentous actin. Neurons were treated for 9 hr with latrunculin A (+LAT) to depolymerize actin, treated for 45 min with TPA as indicated (+TPA), fixed, and immunolabeled for CaMKIIα or NR1 (green) and synapsin (Syn; red). a, b, In latrunculin-treated neurons, CaMKII was diffusely localized, and activation of PKC was unable to induce synaptic accumulation. c, d, In latrunculin-treated neurons, NR1 was clustered at synaptic and nonsynaptic sites. PKC activation induced a loss of synaptic and nonsynaptic clusters and a redistribution to a dispersed plasma membrane-associated pattern even in the absence of detectable F-actin. Boxed regions in a–d are shown below at higher magnification. Scale bar, 10 μm.