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Binding of Myo1c–calmodulin probes to isolated, unfixed hair bundles. Hair bundles were isolated in agarose, permeabilized with saponin, and then labeled with phalloidin and T701. No fixation was used. Left, Actin (phalloidin);middle, T701-Myo1c; right, combined actin (red) and T701 (green). Hair-bundle labeling by T701 was pronounced, particularly at stereociliary tips (arrow). Scale bar, 10 μm.
