Binding of Myo1c neck fragments to hair-cell receptors. A–C, Confocal sections of sacculi at hair-bundle tips (left) and pericuticular necklace (right). Green, N1234, N123, or N12 binding; red, actin. N12 labeling of stereociliary tips was diminished compared with N1234 or N123. D, Coomassie-stained SDS-PAGE gel showing purified complexes of calmodulin (CaM) with T701, N1234, N123, and N12. Molecular mass markers of 75, 50, 35, 25, and 15 kDa are indicated to theleft. E, IQ2 peptide (25 μm) does not affect T701 (230 nm) labeling.Top, Sterociliary tips; bottom, pericuticular necklace. Scale bar: A–C, E, 10 μm.