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. 2002 Apr 1;22(7):2451–2459. doi: 10.1523/JNEUROSCI.22-07-02451.2002

Fig. 7.

Fig. 7.

A, The number of MyT1 mRNA-expressing cells during remyelination was assessed by emulsion autoradiography. MyT1-positive nuclei were defined as nuclei containing >20 silver grains clustered over a single nucleus. The field illustrated is taken from an EB-induced lesion and contains five MyT1-positive nuclei (arrows). Scale bar, 5 μm.B, The changes in MyT1 ROD observed were compared with changes in the absolute number of MyT1-positive nuclei by correlating the total number of cells with the ROD × lesion area for each animal. These data are significantly correlated with one another. In addition, after linear regression (solid line), all data points fall within the 95% prediction intervals (dashed line). The data indicate that the ROD measurements (Fig.6B) reflect changes in the density of MyT1 mRNA-expressing cells.