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. 2002 Apr 1;22(7):2679–2689. doi: 10.1523/JNEUROSCI.22-07-02679.2002

Fig. 1.

Fig. 1.

BrdU-positive cell number is increased in the main olfactory bulb from adult enriched mice. a, BrdU-labeling protocol. The quantification of neurogenesis (survival of BrdU-labeled cells) was done by counting BrdU-labeled cells at 20 d after BrdU injection. All animals received four injections of BrdU at 20 d after being assigned to the two experimental groups (day 0).b, Photomicrograph of a coronal section of the main olfactory bulb displaying the different layers. c–f, BrdU-IR cells in the GCL (c, e) and in the GL (d, f) of standard (c, d) and enriched (e, f) mice. g, Total number of BrdU-IR cells per millimeters cubed throughout the rostrocaudal axis of the olfactory bulb (n = 5 for both groups) 3 weeks after injection. h, Mean number of BrdU-IR cells per millimeters cubed. *p < 0.05 with a Student'st test. Scale bars: b, 300 μm;c–f, 20 μm. ONL, Olfactory nerve layer; GL, glomerular layer;EPL, external plexiform layer; MCL, mitral cell layer; IPL, internal plexiform layer;GCL, granule cell layer.