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. 2002 May 1;22(9):3553–3567. doi: 10.1523/JNEUROSCI.22-09-03553.2002

Fig. 2.

Fig. 2.

Northern and Western blot analysis.A, Northern hybridization with the C-terminalcommon probe on mRNA of adult rat tissues revealednogo-A (4.6 kb) to be strongly expressed in brain, spinal cord (sc), and optic nerve (on). Low levels of nogo-A mRNA were found in DRG, sciatic nerve (scn), testis, and heart. Note that for DRG and sciatic nerve, less mRNA was loaded than for the other tissues.nogo-B mRNA (2.6 kb) was high in the CNS but was also detected in DRG, sciatic nerve, lung, and kidney and at lower level in testis, liver, and spleen. Strong expression of nogo-C(1.7 kb) was observed in spinal cord, brain, optic nerve, and skeletal muscle. In sciatic nerve, heart, liver, spleen, and kidney, expression was lower. For loading control, the blot was reprobed with a glyceraldehyde-3-phosphate dehydrogenase riboprobe. B, In the adult rat, Nogo-A (190 kDa) was strongly expressed in brain and spinal cord, as revealed by AS 472 (and AS Bruna and AS Bianca; data not shown). Apart from the nervous system, Nogo-A was only expressed in detectable amounts in testis and heart. The band present in liver (asterisk) was also detected by preimmune sera and secondary antibody only and represents therefore an unspecific signal. AS Bianca showed expression of Nogo-B (55 kDa) in brain, spinal cord, testis, heart, lung, liver, spleen, and kidney. AS 818 detected a strong Nogo-C band at 25 kDa in skeletal muscle. The smallest Nogo isoform was also present in brain and heart, 40 and 5%, respectively, of the amount present in skeletal muscle, as revealed by densitometric analysis on Western blots with the same amount of total protein loaded.