Table 1.
ED50 values of DOC, DHDOC, and THDOC in seizure and motor toxicity tests in mice
| DOC (mg/kg) | DHDOC (mg/kg) | THDOC (mg/kg) | |
|---|---|---|---|
| Seizure tests | |||
| PTZ | 84 (68–104) | 26 (21–32) | 19 (12–28) |
| Picrotoxin | 97 (77–122) | ND | ND |
| DMCM | 88 (62–125) | ND | ND |
| MES | 108 (35–166) | 58 (48–71) | 48 (35–66) |
| Screen test | 73 (57–94) | 27 (20–36) | 57 (37–90) |
DOC was administered 30 min before testing; DHDOC and THDOC were administered 15 min before testing. In the seizure tests, mice that failed to show clonic spasms during a 30 min period after injection of the chemoconvulsant were scored as protected. ED50 values were determined according to the Litchfield and Wilcoxon procedure. Numbers in parentheses are 95% confidence intervals. ND, Not determined. DOC (≥300 mg/kg, i.p.) had no protective activity against seizures induced by strychnine (1.3 mg/kg, s.c.), kainic acid (32 mg/kg, s.c.), 4-aminopyridine (13.3 mg/kg, s.c.), and NMDA (257 mg/kg, s.c.).