VGLUT2 mRNA in the NK1R-ir neurons of the VRG.A, VGLUT2 mRNA is present in the inferior olive (IO) but absent from the pyramidal tract (pyr). B, VGLUT2 mRNA is absent from the hypoglossal motor nucleus (XII).C, VGLUT2 mRNA is present in the pre-BötC (bregma −12.4 mm) but absent from nucleus ambiguus (NA).D, VGLUT2 mRNA is present in the rVRG (bregma −13.0 mm). NK1R-ir neurons shown under fluorescence illumination inE contain VGLUT2 mRNA as seen under bright-field inF (arrows show two double-labeled neurons). Scale bars (shown in A):A–D, 100 μm; (shown inF): E, F, 20 μm.