Fig. 4.

Confocal reconstruction of Purkinje cells in the absence and presence of Gö6976. Three-dimensional reconstructions of confocal sections were obtained using ScanWare software (Leica TCS 4D) of P3 (A–F), and P5 mice (G–K) in the absence (A–C, G, H) or presence (D–F, I–K) of Gö6976. The arrowheads in A–C, H, I, K point to the primary branching points; thearrows in D, E, J and thearrowhead in E point to axon emerging points. PKC inhibitors exert important qualitative effects on dendritic and axonal arbors (compare A–C with D, Ffor P3; G, H with J, K for P5). Magnification: A, D, G, I, 10×; E, 20×;B, C, F, H, J, K, 100×.