Fig. 6.

Axonal regeneration of Purkinje cells in the presence or absence of Gö6976. Coculture of the dorsal regions of wild-type E18 (A, B), P3 (C, D), P5 (E–G), and P7 (H, I) mouse cerebellar slices with the dorsal region of split P10 cerebellar slices taken from a CaBP-null mutant in the absence (A, C, E, F, H) or presence (B, D, G, I) of Gö6976 is shown. The dashed lines represent the borderlines between the two cocultures. Note that the number of regenerative axons decreases with age in the absence (compare A, C, F, H) or presence (B, D, G, I) of Gö6976. For each time point, the regenerative capability is always stronger in the presence of Gö6976 than in its absence (compare A withB, C with D,F with G, H withI). E, Enlargement ofF (arrows point to the same cell inE, F). Scale bar: A–D, F–I, 150 μm; E, 22.5 μm.