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. 2002 May 1;22(9):3663–3672. doi: 10.1523/JNEUROSCI.22-09-03663.2002

Fig. 4.

Fig. 4.

CRE-mediated transcription is induced in projection neurons and interneurons in the nucleus accumbens during morphine withdrawal. Double-labeling for β-gal immunoreactivity and for preproenkephalin (PPE) (A) and preprodynorphin (PPD) (B) mRNA revealed prominent colocalization of β-gal with both neuropeptides in mice undergoing withdrawal (arrows indicate double-labeled cells, 60×). C, Confocal images of double immunofluorescently labeled sections from mice in withdrawal revealed considerable cellular colocalization between β-gal (green, nuclear, 20×) and the interneuron marker calbindin (red, cytoplasmic, confocal image 20×). In contrast, no colocalization was observed between β-gal (green, nuclear) and markers of two other interneurons, choline acetyltransferase (ChAT) (D, green, cytoplasmic, 20×;asterisk indicates cholinergic interneuron) and parvalbumin (E, green, cytoplasmic, 60×;asterisk indicates parvalbumin+ interneuron). β-gal immunoreactivity also did not colocalize with the glial marker S-100 (F, red, cytoplasmic, 20×;asterisk indicates glial cell). Results are representative of the following mean number of β-gal+ cells counted in each of three animals: 28 cells per animal for PPE; 24 cells per animal for PPD; 45 cells per animal for calbindin; 13 cells per animal for ChAT; 14 cells per animal for parvalbumin; and 11 cells per animal for S-100.