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. 2002 May 1;22(9):3663–3672. doi: 10.1523/JNEUROSCI.22-09-03663.2002

Fig. 5.

Fig. 5.

CRE-mediated transcription is altered in monoaminergic nuclei during morphine withdrawal. Mice received morphine or sham pellets over 5 d followed by naltrexone. The number of cells expressing β-gal was counted for each region on two to four hemisections in withdrawal mice (C, G, K) and naltrexone controls (B, F, J).Arrows indicate one example of a β-gal+ cell in (B, F, K). For the VTA (A–D) and LC (I–L), quantitation focused on areas that stained for tyrosine hydroxylase at bregma levels −3.16 and −5.40 mm, respectively (areas highlighted byblue box in A andI). For the DRN (E–H), quantitation focused on the B6 and B7 regions at bregma level −4.36 mm, which showed staining for serotonin (area highlighted byblue box in E). There was a significant decrease in the number of β-gal+ cells per unit area in both the VTA (D) and the DRN (H) in mice undergoing withdrawal compared with naltrexone controls (n = 8–10 animals in each group;p < 0.05 by t test). There was a strong trend for an increase in the number of β-gal+ cells per unit area in the LC (L) in the withdrawal group compared with naltrexone controls (n = 10 for each group; p < 0.09).