Fig. 7.

ChAT immunoreactivity in an LA neuron.A, Spike evoked in an LA neuron, showing the characteristic long-lasting afterhyperpolarization. The baseline potential was −64 mV, maintained by continuous injection of −0.04 nA.B, Biocytin fill of this neuron, visualized with Cy2-conjugated streptavidin and acquired with a confocal microscope. Scale bar, 25 μm. C, ChAT immunostaining in this neuron visualized with a Cy5-conjugated secondary antibody, suggesting that it is cholinergic. This neuron was recorded from a zebra finch brain slice.