Fig. 3.

MKK4 is activated in Tg2576/PS1^P264L cortex. A, Thetop demonstrates 100 μg of cortical protein per sample stained with an antibody against phosphorylated MKK4 (phospho-thr261). This site is phosphorylated by MAPKK kinases and indicates the presence of more activated MKK4 in 7 and 12 month cortical samples compared with 12-month-old wild-type littermates (WT 12). Thebottom demonstrates total MKK4 using the same blot.B represents the active fraction of MKK4 from a density ratio of phospho-MKK4 to total MKK4. Six samples per group, each assayed in triplicate by immunoblot. *p ≤ 0.05 indicates significant differences.