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Measurement of resting [Ca2+]i at early time points after slice isolation. Brain slices were loaded with fura-2 AM after a minimal re-equilibration period, and all [Ca2+]i recordings were performed within 60–90 min after brain dissection. The numbers on the graph represent the numbers of neurons in each respective age group. There was no statistical significant difference between the mean resting [Ca2+]i value for any of the age groups (one-way ANOVA).
