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. 2002 Dec 15;22(24):10514–10518. doi: 10.1523/JNEUROSCI.22-24-10514.2002

Fig. 2.

Fig. 2.

Association of actin and CSP24 in components of the CSP. A, 32PO4 incorporation into proteins in a sample of lysates from preparations exposed to 10−4m 5-HT (lane 1).32PO4 incorporation into anti-CSP24 immunoprecipitates revealed a 44 kDa protein band that coprecipitated with CSP24 and the 29 kDa splice variant of CSP24 (lane 2). B, Anti-actin antibody recognized a single 44 kDa protein band on Western blots (lane 1). The detection of the 44 kDa protein was blocked by preabsorption exposure with the peptide antigen (blocking peptide) on the Western blot (lane 2). C, Coimmunoprecipitation of actin and CSP24 shown by the blot generated from a nonreducing gel of anti-CSP24 immunoprecipitates probed with anti-actin antibody. Western blot probed with anti-actin antibody detected a 44 kDa protein band (actin) from anti-CSP24 immunoprecipitates without exposure to a reducing agent (β-mercaptoethanol). The dark bands at higher molecular weight represent the antibody used for immunoprecipitation.