Fig. 4.

One-trial conditioning regulates CSP24 phosphorylation in immunoprecipitates of the CSP (eyes and proximal optic nerve). A, ³²PO₄incorporation into CSP24 in lysates from the in vitroconditioned group and unpaired controls. B,³²PO₄ incorporation into CSP24 of the supernatants from the same groups as shown in A. Immunoprecipitation with anti-CSP24 reduced labeling of CSP24 in the supernatants of all groups compared with the lysates. C,³²PO₄ incorporation into anti-CSP24 immunoprecipitates from the conditioned group and unpaired controls.D, Examples of Coomassie blue-stained 44 kDa protein bands from the CSP, P, and CP lysates of conditioned and unpaired controls. The examples show approximately equal loading of protein for the experimental and control groups. E, Group data showing mean ± SE. C/UP ratios of densitometric measurements for CSP24 from the immunoprecipitates. One-trial conditioning resulted in a significant increase in ³²PO₄ incorporation in the immunoprecipitates from the CSP compared with the unpaired control group CSP; *p < 0.003. However, the P and CP immunoprecipitates were not significantly different from their respective unpaired controls or each other.