Fig. 6.

Voltage-gated calcium channels, particularly the P/Q type, are required for spontaneous firing in Purkinje neurons.a, In a tonically firing Purkinje neuron, bath application of CdCl₂ (100 μm) to block voltage-gated calcium channels caused the cell to burst and then go silent. b, Block of voltage-gated calcium channels by application of CdCl₂ (100 μm) also inhibited firing in a Purkinje neuron that expressed the trimodal pattern of activity. c, Bath application of 1 μmωCgTx MVIIC to a tonically firing Purkinje neuron caused the cell to burst and then go silent. d, Application of ωCgTxMVIIC (1 μm) to a Purkinje neuron that expressed the trimodal pattern inhibited firing. e, Bath application of 100 nm ωAga IV-A to a tonically firing Purkinje neuron initially increased the firing rate and then caused it to burst and go silent. f, Application of 100 nm ωAga IV-A to a Purkinje neuron with the trimodal pattern caused it to burst and go silent.