Fig. 12.

C121Wβ1 does not exert a dominant-negative effect on cell adhesion in Drosophila S2 cells. Stable S2β1 cell lines (Malhotra et al., 2000) were transfected with increasing amounts of C121Wβ1 plasmid, as indicated above, using Fugene reagent. Selection (250 μg/ml hygromycin) was started 48 hr after transfection and continued for 30 d. Transfected or untransfected S2 cells were induced in the presence of 0.7 mm CuSO₄, and then aggregation was induced by rotary shaking. Cells were viewed with a phase-contrast microscope. Representative fields of view are presented for 0 (S2β1), 1, and 5 μg of transfected C121Wβ1 plasmid, respectively. Untransfected S2 cells are presented for comparison. Scale bar, 100 μm.