Fig. 2.
Wild-type β1 and C121Wβ1 associate with human Nav1.3 α subunits in CNahIII-12 cells. Coimmunoprecipitation experiments in two different CNahIII-12-derived cell lines, one stably coexpressing the human Nav1.3 α subunit and the human β1 subunit (right-hand blot) and the other stably coexpressing Nav1.3 and C121Wβ1 (left-hand blot). In each experiment, sodium channels were immunoprecipitated from solubilized membranes using an anti-Nav1.3 antibody and then probed using an anti-β1 polyclonal antiserum.