Fig. 6.
StAR is expressed in steroidogenic cells in the brain. A–C, P450scc immunoreactivity was detected in the cerebellum (A), pons (B), and cerebral cortex (C). D, E, As with StAR, P450scc (reddish brown) colocalized in individual cells with NeuN (bluish gray), shown in the cerebral cortex (D), and GFAP, as in the lateral hypothalamus (indicated by thick arrow; cell lacking P450scc indicated by an asterisk,E), indicating expression in both neurons and glia.F, StAR (bluish gray) and P450scc (reddish brown) immunoreactivity colocalized in cells within the cerebral cortex. G–J, Nuclear-excluded immunofluorescence (by DAPI stain, G) established for StAR (H, red) and P450scc (I, green) mitochondrial-type colocalization (visualized in three channels inJ), using DAPI, Texas Red, and FITC filters. Scale bars: A, 100 μm; B–D, 50 μm;E, F, G–J, 25 μm.