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TNF receptor gene transcription in hippocampal neurons. Single-cell RT-PCR of cultured primary hippocampal neurons. Gene transcripts for TNFRI and TNFRII were detected in the majority of neurons. Co-amplification of gene transcripts for GAPDH served as a control. M, Molecular weight marker ΦX174/HaeIII; C, negative PCR control;N1–N9, samples of single neurons.
