Fig. 9.

Difference in soma size is not evident during early postnatal development. Confocal images showing double immunolabeling with SMI-32 (green) and GABA (red) from the dorsolateral prefrontal cortex (A, B) and parietal cortex (C, D) of P7 wild-type (A,C) and knock-out (B, D) mice. Although we have not noticed any change in the soma size of the pyramidal neurons (stained in green with SMI-32) between the genotypes at this stage, note that there are fewer SMI-stained neurons and processes in the knock-out mice (B,D). In the parietal cortex, although we can see pyramidal cells arranged in columns with their extended processes in the wild-type mice (C), such cells and processes are not noticeable in the knock-out mice (D). Note that the GABA interneurons (stained in red) are not changed between the genotypes. Scale bar, 50 μm.