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. 2002 Feb 1;22(3):674–683. doi: 10.1523/JNEUROSCI.22-03-00674.2002

Fig. 7.

Fig. 7.

No alteration in the DEAE column elution profile of cofactor-dependent PKC activity after the induction of LTP.A, Induction of LTP. Stable baseline responses of the fEPSP slope were recorded in hippocampal slices for 20 min before the delivery of HFS as indicated by the arrow. Responses were recorded for either 2 min (filled circles,n = 5) or 45 min (open squares,n = 5) after the final train of HFS. Error bars are SEM for the indicated number of determinations. B, C, Elution profile of cofactor-dependent PKC activity from either control slices (open circles in B, open squares in C) or slices taken 2 min (filled circles in B) and 45 min (filled squares in C) after the final train of LTP-inducing HFS. Either 2 or 45 min after the final train of HFS the slices were frozen, and the area between the stimulating and recording electrodes was dissected and homogenized. The soluble fraction of the homogenates was applied to mini-DEAE columns, and PKC was eluted with either 0.1 or 0.25 m NaCl (arrows). Cofactor-dependent PKC activity was measured as described in Materials and Methods.